Total RNA extraction is a fundamental step in numerous molecular biology investigations. This process involves the careful separation of total RNA from diverse biological matrices, such as tissues, cells, and body fluids. The aim is to obtain high-quality RNA that is undegraded from contaminating substances. A variety of techniques are available f

RNA is a fragile molecule that is easily degraded by enzymes called RNases. Therefore, it is essential to carefully handle RNA samples and use aseptic techniques during isolation and purification processes. There are several methods available for isolating RNA from various sources, including {phenol-chloroform extraction, column chromatography, and